Bio-Ag Consultants & Distributors Inc.

Swine....Hog Trial 1 - Efficacy Testing of EnzoLac

We are very proud of the new product, Enzolac, that we are producing on-site. Enzolac is now included in all our Bio-Ag Mineral Premixes reinforcing that Bio-Ag Premixes are a superior product in the market today. We believe that we have found, developed and are producing one of the best lactic acid/lactobacillus/enzyme products in the world. The following information is available from a recent hog study using Enzolac and a competitor product.

HOG TRIAL....Porcine Blood Profile .  Competitor Bio-Ag Enzolac . Test  A  B  C  D  Reference Interval  Beta-Hydroxybutyrate  81  2.7  79  56 . .  umo/L  Haptoglobin  0.43  0.55  0.30  0.25 . .  g/L  Calcium Serum M2 Hemolysis  2.62  2.58  2.49  2.65  1.80  2.90  mmol/L  Phosphorus  2.89  2.97  2.74  2.84  1.6  3.4  mmol/L  Magnesium  0.90  0.80  0.80  0.90  0.8  1.6  mm0l/L  Sodium  141  142  143  145  140  150  mmol/L  Potassium  5.3  6.2  4.5  4.8  4.7  7.1  mmol/L  Chloride  96  99  102  100  99  105   mmol/L  Total Protein  71  74  63  70  61  81  g/L  Albumin  39  40  35  43  27  39  g/L  Globulin  32  34  28  27  34  43  g/L  A:G Ratio  1.22  1.18  1.25  1.59  0.5  1.2 .  Urea  8.0  6.9  4.1  4.4  3.0  8.5  mmol/L  Creatinine  107  88  90  99  90  240  umol/L  Glucose  5.2  5.9  5.3  4.9  3.6  5.3  mmol/L  Cholesterol  2.25  2.24  2.36  2.25  2.00  5.00   mmol/L  Alkaline Phosphatase  204  125  122  171  0  500  U/L  Gamma-GT  16  36  27  38  10  400  U/L  AST  38  38  26  20  25.0  60.0  U/L  CK  309  504  666  570  0  800  U/L  GLDH  2  1  1  1  0  25  U/L  Total Bilirubin  1  1  2  1  0  4  umol/L  Conjugated Bilibrubin  0  0  0  0  0  4   umol/L  Free Bilirubin  1  1  2  1  0  8   umol/L  Calculated Osmo  285  288  284  288  280  320   mmol/L  GRADES . . . . . . .  Complete Blood Count Panel .   Competitor   Bio-Ag Enzolac . Test  A  B  C  D  Reference Interval WBC  16.1  18.3  24.1  20.9  11.0  22.0  10^9/L  RBC  6.8  6.4  5.9  7.0  5.0  8.2  10^12/L  Hb  128  118  106  125  99  158  g/L  HCT  0.37  0.36  0.32  0.39  0.32  0.50  L/L  MCV  55  56  54  56  51  68  fL  MCH  19  18  18  18  17  22  pg  MCHC  342  327  330  317  300  341  g/L  RDW  16.8  17.1  17.5  16.9 . .  %  Platelets  152  202  376  264  325  700  10^9/L  MPV  8.8  9.1  8.4  8.3 . .  fl  T.S.Protein Plasma Hemolyzed M1  71  74  60  66  50  80  g/L  Seg Neutrophil Count  7.41  4.94  12.77  6.48  4.0  9.5  10^9/L  Band Neutrophil Count  0.16 . . .  0.0  0.6  10^9/L  Lymphocyte Count  6.95  10.98  10.36  12.33  6.0  12.6   10^9/L  Monocyte Count  1.61  2.20 . . . . .  Eosinophil Count  -  0.18 . 0.42   1.0  2.2  10^9/L  Basophil Count  -  -  -  0.21  0.0  0.3   10^9/L  Polychromasia  occ .  1-2  occ . .  /100X  Anisocytosis  occ  3+  2+  2+ . . .  Poikilocytsis  2+  2+  2+  2+ . . .  Shift Platelets  -  1+  occ  1+ . . . Graphs of Table Information

Summary of Hog Trial

If we look at this test of the White Blood Cells (WBC), we will notice that the competitor had an average count of approximately 17. If we look at Bio-Ag's Enzolac we can see that the average was close to 22. We know from looking at the comparison, that the Red Blood Cell (RBC) average was close to the same thing. The hemoglobin was also reasonably close.

In the breakdown of the different white blood cells, we notice that one of the most important white blood cells, the neutrophil was much higher on average, running approximately 9.6 on the Enzolac trial, compared with approximately 6.1 on the competitor's hogs. On the mineral comparisons, we can see that many things are reasonably constant, however one thing that stands out very clearly is the amount of blood urea nitrogen. The blood urea nitrogen averaged 7.5 for the competitor and only 4.3 on Bio-Ag hogs which is a clear indication that there is far less free protein converting into ammonia floating around in the bloodstream. This means that the hogs are making extremely efficient use of the feed they are consuming and are converting a lot of this to more good muscle mass.

Aside from that, not very many of the other numbers carry much significance however the grades on the competitor's products were 105.1 and on the Enzolac were 106.5. When weights were adjusted, many of the Bio-Ag hogs were actually overweight and the farmer was penalized because he did not think the hogs were heavy enough. Indeed, they were overweight. Taking the weight of these hogs into consideration and subtracting and figuring the feedback, it took approximately 5% less feed to put these Bio-Ag hogs to market compared to the competitor.

 

The Spleen

The spleen is critically important to the health of the animal or human being.

The spleen is the largest collection of reticuloendothelial cells in the body. It is composed of spongelike tissue (splenic pulp) consisting of lymphatic tissue differentiated into white pulp and pulp infiltrated with red blood cells (red pulp). If bone marrow is damaged, the spleen can function to produce various blood cells. Smooth muscle and elastic tissue fibers enable the spleen to contract and discharge blood cells into circulation. The spleen is the major organ that transforms food into energy and it also assures that food will make healthy blood. Another major role of the spleen is to regulate blood predominantly in keeping it in the vessels and preventing its depletion via excessive extravasation. Western medicine acknowledges that the spleen is a producer of antibodies and special lymphocytes and is overall, a major partner of the bodily Pentagon - a powerful reliculoendothelial system that represents a chief defense against infections, potential allergens and malignancies.

Spleens 168 and 169 belong to the competition, both of these spleens were between 1/2 to 3/4" thick. Note the narrowness of 168 and also the shortness of 169. If you look at Bio-Ag's174 and 175 you will notice immediately the wideness which is associated with better health. Spleens 174 and 175 are between 1/4 and 3/8 inch thick indicating far less inflammation or pressure on the spleen. This is backed up by the blood test which shows half the amount of blood urea nitrogen and also shows a higher level of white blood cell activity. This would also make sense due to the fact that the hogs consumed 5% less feed. The spleens 174 and 175 were also a deeper darker bluish purple color strongly indicative of an exceptionally healthy spleen.

 

Manure Analysis Report	HOG TRIAL....Manure Analysis Report Taken in the Beginning, Middle and at the End of Test Time .  Competitor Enzolac  July 14  Aug.18  Jan.15   July 14   Aug.18 Jan.15 Dry Matter   %  24.8  30.7 28.0  26.2  26.7 26.0 Total Nitrogen  %  .86  1.20 1.04  1.11  1.23 0.81 Ammonium N  ppm  986  1451 1732  1190  1586 920 Phosphorous  %  0.64  0.79 0.71  0.59 0 .71 0.59 Potassium  %  0.23  0.33 0.35  0.39  0.41 0.37 Magnesium  %  0.27  0.26 0.23  0.25  0.25 0.18 Calcium  %  0.96  1.31 0.53  0.71  0.90 0.42 Sodium  %  0.10  0.07 0.13  0.07  0.06 0.09 Iron  PPM  734  599 646  448  490 320 Manganese  PPM  146  146 122  53  55 54 Copper  PPM  265  267 245  12  14 13 Zinc  PPM  346  345 279  79  87 65 Nutrient Value  Nitrogen (N)  lb/ton  3.5  5.0 5.2  4.4  5.3 3.3  Phosphate(P205)  LB/ton  11.8  14.5 13.1  10.9  13.1 10.9  Potash (K20)  LB/ton  5.0  7.1 7.6  8.4  8.9 8.0 Graphs of Table Information
Manure Analysis Report....The other thing of significance was the manure analysis report. You can see these tests were taken part way through the trial and phosphorous was significantly lower with the Bio-Ag hogs. The competitor had 0.64 and .79 compared with 0.59 and 0.71 on the BioAg program. When we get to the heavy metal portion, iron, manganese, copper and zinc showed a dramatic difference in terms of what is passing through in the manure. Some of the most significant things on the Bio-Ag hogs were a much better bone marrow mass and far less iron-734 (Competitor) versus 448 (Bio-Ag) and 599 versus 490. Manganese was 146 competitor versus 53 and 55. Copper was the most significant element of all in that we could see 265 to 267 on the competitor versus 12 and 14 with Bio-Ag. Part of the explanation for this is that Bio-Ag uses far more chelated material so therefore has to put a lot less toxic substances such as copper in the premix. This copper in a chelate form is much more available to the hog so therefore the level of copper is diminished that the farmer hauls back onto his field in terms of toxicity problems in his soil. As an adjunct to this, many states in the Southeast United States now have such high levels of copper toxicity from their hog manure that much of this land can no longer be properly farmed. The zinc is also significant. In the competitor, zinc averaged 345 and only 83 for BioAg's program. We also notice that the nutrient value seemed to have a slight bit more nitrogen but this nitrogen was bound up in a much more readily available form in this manure and also seemed to release more slowly in the field. The phosphate is also significant-11.8 & 14.5 for the competitor versus 10.9 & 13.1 for the Bio-Ag program. This means less phosphate contamination for our waterways. Potash levels were slightly higher. This is something we have not been able to fully explain at this point in time other than the sodium:potassium ratios were much better in the body and the hog had to excrete some of the extra potash.

 

Analysis of Table Information:

Phosphorous was significantly lower with the Bio-Ag hogs. The competitor had 0.64, .79 and 0.71 readings, compared with 0.59, 0.71 and 0.59 readings on the Bio-Ag program.

When we get to the heavy metal portion, iron, manganese, copper and zinc showed a dramatic difference in terms of what is passing through in the manure. Some of the most significant things on the Bio-Ag hogs were a much better bone marrow mass and far less iron-734, 599 and 646 readings (Competitor) versus 448, 490 and 320 (Bio-Ag)

Manganese levels were 146 ppm for two samples and 122 ppm for the third, while the results for the Bio-Ag barn gave results of only 53, 55, and 54 ppm.

Copper was the most significant element of all in that we could see 245 to 267 on the competitor versus 12 to14 with Bio-Ag.

Part of the explanation for this is that Bio-Ag uses far more chelated material so therefore has to put a lot less toxic substances such as copper in the premix. This copper in a chelate form is much more available to the hog so therefore the level of copper is diminished that the farmer hauls back onto his field in terms of toxicity problems in his soil. As an adjunct to this, many states in the Southeast United States now have such high levels of copper toxicity from their hog manure that much of this land can no longer be properly farmed.

The zinc is also significant. In the competitor, zinc readings were 346, 345 and 279, with Bio-Ag's program readings being only 79, 87 and 65.

We also notice that the nutrient value seemed to have a slight bit more nitrogen but this nitrogen was bound up in a much more readily available form in this manure and also seemed to release more slowly in the field. The phosphate is also significant with lower levels on all three tests for the Bio-Ag program. This means less phosphate contamination for our farms and our waterways.

Potash levels were slightly higher. This is something we have not been able to fully explain at this point in time other than the sodium: potassium ratios were much better in the body and we think this meant the hog had to excrete some of the extra potash.

 

Results of Manure Testing for Salmonella and E.coli done by Agri Foods Labs.

The Enzolac Group of Hogs was 0.0 C.F.U. (Colony Forming Units) for Salmonella and 90,000 C.F.U. for E.coli.

The competitor was 0.0 C.F.U. for Salmonella, but was 2,000,000 C.F.U. for E.coli.